HIGH PRESSURE LIQUID Chromatograph (HPLC-DOUBLE PUMP)

Model No: AGN-3202
High-Pressure Liquid Chromatograph (HPLC) with a Double Pump Binary Gradient System is a precision analytical instrument designed for high-resolution separation, identification, and quantification of components in complex mixtures. This system is equipped with two high-pressure solvent delivery pumps, a multi-wavelength UV detector, a manual sample injection valve (Rheodyne 7725i), and a high-quality USA-made LC column, all integrated with powerful workstation software for data acquisition and analysis.

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Technical Specifications

  1. Binary Gradient System (Double Pump Configuration)
  • Two independent high-pressure solvent delivery pumps allow for binary gradient elution, enabling flexible and reproducible solvent mixing.
  • Ensures accurate and stable flow rates for high-resolution chromatographic separations.
  • High chemical resistance and low pulsation flow for enhanced reproducibility.
  1. Multi-Wavelength UV Detector
  • Wavelength Range: 190–700 nm – covers full UV to visible spectrum.
  • Wavelength Accuracy: ±1 nm – ensures reliable detection of peaks at precise wavelengths.
  • Spectral Bandwidth: 8 nm – suitable for a wide range of analytes.
  • Flow Cell Volume: 8 µL with 10 mm optical path length – balances sensitivity with efficiency, suitable for micro to analytical scale.
  1. Sample Injection Valve
  • Model: Rheodyne 7725i – a durable, high-precision manual injector.
  • Compatible with standard 20 µL sample loops (other volumes optional).
  • Easy-to-use front-panel access for reproducible manual injections.
  1. LC Column
  • Origin: USA-made – ensures high quality and performance.
  • Column specifications not detailed but typically includes C18 or C8 for reversed-phase applications.
  1. Workstation Software
  • Full-featured software for system control, real-time monitoring, data acquisition, chromatogram analysis, report generation, and method storage.
  • Allows customization of gradient programs, detector settings, and integration parameters.

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